Efficient Production of Secreted and Active Invertase
نویسندگان
چکیده
منابع مشابه
Two differentially regulated mRNAs with different 5' ends encode secreted with intracellular forms of yeast invertase.
The SUC2 gene of yeast (Saccharomyces) encodes two forms of invertase: a secreted, glycosylated form, the synthesis of which is regulated by glucose repression, and an intracellular, nonglycosylated enzyme that is produced constitutively. The SUC2 gene has been cloned and shown to encode two RNAs (1.8 and 1.9 kb) that differ at their 5' ends. The stable level of the larger RNA is regulated by g...
متن کاملSecretion-defective mutations in the signal sequence for Saccharomyces cerevisiae invertase.
Nine mutations in the signal sequence region of the gene specifying the secreted Saccharomyces cerevisiae enzyme invertase were constructed in vitro. The consequences of these mutations were studied after returning the mutated genes to yeast cells. Short deletions and two extensive substitution mutations allowed normal expression and secretion of invertase. Other substitution mutations and long...
متن کاملDate Wastes as Substrate for the Production of a-Amylase and Invertase
Background: In Algeria the date wastes production is estimated at least 85.000 tones. Date wastes is an economic source of carbohydrates for conversion to industrial enzymes because it is readily available and relatively low priced. Objective: The aim of the present study was to investigate the potential of using date wastes as a substrate for the production of a-amylase and invertase. Materia...
متن کاملMolecular and functional characterization of an invertase secreted by Ashbya gossypii.
The repertoire of hydrolytic enzymes natively secreted by the filamentous fungus Ashbya (Eremothecium) gossypii has been poorly explored. Here, an invertase secreted by this flavinogenic fungus was for the first time molecularly and functionally characterized. Invertase activity was detected in A. gossypii culture supernatants and cell-associated fractions. Extracellular invertase migrated in a...
متن کاملAn Efficient Genome-Wide Fusion Partner Screening System for Secretion of Recombinant Proteins in Yeast
To produce rarely secreted recombinant proteins in the yeast Saccharomyces cerevisiae, we developed a novel genome-wide optimal translational fusion partner (TFP) screening system that involves recruitment of an optimal secretion signal and fusion partner. A TFP library was constructed from a genomic and truncated cDNA library by using the invertase-based signal sequence trap technique. The eff...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
دوره شماره
صفحات -
تاریخ انتشار 2017